Composite

Part:BBa_K5286003:Design

Designed by: Marten Meckelburg   Group: iGEM24_TUM-Straubing   (2024-10-01)


CAHS107838+His Tag for protein purification


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 702
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 702
    Illegal NotI site found at 708
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 702
    Illegal XhoI site found at 559
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 702
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 702
    Illegal AgeI site found at 761
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The basic BBa_K5286000 coding sequence part (CAHS107838 from Ramazzottius varieornatus) was assembled to the pET-16b vector without scars via Gibson Assembly.


Source

This part was assembled by the 2024 iGEM team from TUM campus Straubing. The coding sequence part (CAHS107838 from Ramazzottius varieornatus) was assembled into the pET-16b vector, which includes the HisTag.

References